Instrumentation

The instruments listed on this page are located on the Durham campus of the University of New Hampshire. In addition to those listed below, the UIC maintains a database of major analytical instruments on campus, the Scientific Instrumentation Inventory (SII).

Scientific Instrumentation Inventory (SII)

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The Knowledge Base contains forms, instruction and training material, minutes, policies, tools and other resources to support your research efforts by topic area.

SCIENTIST Labor Rates

UNH Rate:  $78/hour (1/2-hour minimum)

Non-UNH Academic Rate: $156.00/hour (1/2 hr minimum)

Industry Rate: Contact the UIC

Cytometry

Cytometry is used for the measurement of the characteristics of cells including cell size, cell count, cell morphology, and other characteristics. The UIC’s cytometry instrumentation (analyzer and sorter) is located in a BSL-2 laboratory in Parsons Hall in Room W118A and is available for shared use.

  • Flow CytometerThe Becton-Dickinson FACSCalibur Flow Cytometer (June, 2000 mfr. date) is a four-color, dual-laser, bench-top system capable of cell analysis using forward scatter, side scatter, and detection of fluorescence in four distinct color regions: > 670 nm (deep red), 653-669 nm (red), 564-606 nm (orange), and 515-545 nm (green). The unit has a Mac G4 host computer, and most instrument functions are computer controlled.
  • Sorting Flow CytometerThe Sony SH800Z Sorting Flow Cytometer is a compact, easy to use instrument that performs both cell counting and two-way cell sorting.  The integrated laser engine houses 4 lasers, providing excitation lines of 405 nm, 488 nm, 561 nm and 638 nm.  These lasers are combined and delivered through a single fiber while 6 freeform PMTs allow for the detection of fluorescence signals from any laser based on filter selection.

Electron Microscopy

Electron microscopy is a type of microscopy using electron beams to create an image of a specimen. Electron Microscopy is able to create much higher magnifications and resolution than a light microscope which allows one to much smaller objects in finer detail. The UIC operates both a transmission electron microscope and a scanning electron microscope which are located in Parsons Hall, Room NB17.

Light Microscopy

Light microscopy is a type of microscopy using visible light and magnifying lenses to examine small objects not visible to the naked eye. The UIC operates several varieties of light microscopes which are located in locations on campus including Rudman Hall rooms 157 and 340, Parsons Hall room W118A.

  • LIGHT MICROSCOPY FACILITY The University Instrumentation Center's shared light microscopy facility is located in Rudman Hall 157. Any researchers are encouraged to make use of this facility which includes both upright and inverted fluorescence microscopes as well as a cryo microtome. Training and access to the facility can be provided by the UIC upon request.
  • CONFOCAL MICROSCOPY - NIKON A1R  HDThe NIKON A1R - HD Confocal microscope is available for researchers and offers extremely capable features for analyzing fluorescently labled samples of various thickness and the efficient creation of 3D reconstructions.

  • CONFOCAL MICROSCOPY - ZEISS LSM 510 METAThe Zeiss LSM 510 Meta laser scanning confocal microscope is used primarily by researchers in the biological sciences to image fluorescent probes in cells and tissues. However, confocal microscopes are finding increasing use in non-biological applications as well. 

Mass Spectrometry

Mass spectrometry uses electric and magnetic fields to measure the weight of charged particles from the specimen being analyzed. There are a variety of mass spectrometer capabilities available through the UIC as well as throughout campus and the following represent a small percentage of what is available.

  • THERMO SCIENTIFIC LTQ ORBITRAP XL MASS SPECTROMETERThe Thermo Scientific Orbitrap LTQ XL Mass Spectrometer provides accurate mass measurement (<10 ppm) with high resolution (600,000) and sensitivity (low fetomole to high attomole level).
  • THERMO SCIENTIFIC LTQ XL™ LINEAR ION TRAP MASS SPECTROMETERThe Thermo Scientific LTQ XL™ Linear Ion Trap Mass Spectrometer provides accurate mass measurements (m/z 50-4000) with resolution of 60,000 and high sensitivity (low fetomole to high attomole level). The multiple fragmentation technique—PQD, CID—for routine structural elucidation.
  • THERMO SCIENTIFIC LTQ OBITRAP VELOSThe Thermo Scientific LTQ Obitrap Velos provides exact mass measurements (m/z 50-4000) with resolution of >60,000 and mass accuracy of <3 ppm, or 1 ppm with the use of lockmass. The multiple fragmentation techniques, high collision dissociation(HCD) and collision induced dissociation (CID), enable complete structural information to be obtained through MS and MSanalysis.

SPECTROSCOPY

Spectroscopy refers to the measurement of radiation intensity as a function of wavelength and is used to determine physical characteristics of specimen. Examples of spectroscopy within the UIC include nuclear magnet resonance, X-Ray Photoelectron, and FT-IR spectroscopy to name a few.

  • NUCLEAR MAGNETIC RESONANCE SPECTROSCOPYThe NMR is a powerful tool for the analysis of sequence, conformation, and other molecular attributes of biologically significant molecules, organics, and polymers, and also in the rapidly expanding areas of organometallic and inorganic NMR.
  • X-RAY PHOTOELECTRON SPECTROSCOPYThe Kratos Axis HS XPS (X-ray Photoelectron Spectroscopy) system offers surface analysis, surface chemical mapping, and depth profiling of metallic, semi-metallic, and nonmetallic samples as deep as 1 nm. The sample is evacuated to 10-9 Torr or better, for quality measurements.
  • FOURIER TRANSFORM INFRARED SPECTROMETER (FT-IR)FT-IR is used to obtain an absorption or transmission spectrum of a solid or liquid. An FT-IR spectrometer simultaneously collects data over a wide spectral range.  Multiple 

other instruments

The UIC provides oversight for several other instruments that are available for use by faculty, students, staff and users outside UNH. Many of these instruments are located in non-UIC labs with agreements for use by others. If you don’t see what you need, just inquire with the UIC.

  • POLARIMETERThe Rudolph Autopol III Polarimeter measures the optical activity of a compound at 589 nm (sodium D line).  A solution of known concentration and volume is prepared and inserted into a polarimetry cell (10 cm or 1 cm). 
  • BET SURFACE AREA ANALYZERThe Quantachrome NOVA 2200E BET Surface Area Analyzer can measure surface areas, pore volume, and pore size distribution.

HIGH THROUGHPUT INSTRUMENTS

Several instruments that lend themselves for high throughput processing of specimen are available including an automated cell imaging system, instruments with automated sample changers and high output processing.

  • SCANNING ELECTRON MICROSCOPE WITH FOCUSED ION BEAM (FIB-SEM)A state of the art SEM with Schottky field emission cathode in combination with gallium Focused Ion Beam (FIB) column and Gas Injection System (GIS).
  • X-RAY PHOTOELECTRON SPECTROSCOPYThe Kratos Axis HS XPS (X-ray Photoelectron Spectroscopy) system offers surface analysis, surface chemical mapping, and depth profiling of metallic, semi-metallic, and nonmetallic samples as deep as 1 nm. The sample is evacuated to 10-9 Torr or better, for quality measurements.