The Becton-Dickinson FACSCalibur Flow Cytometer is a four-color, dual-laser, bench-top system capable of cell analysis using forward scatter, side scatter, and detection of fluorescence in four distinct color regions: > 670 nm (deep red), 653-669 nm (red), 564-606 nm (orange), and 515-545 nm (green). The unit has a Mac G4 host computer, and most instrument functions are computer controlled.
The unit has two lasers for exciting fluorochromes: an argon laser, which emits sapphire-colored light at 488 nm, and a red diode laser emitting light at 635 nm. This unit is best suited for the analysis of aqueous suspensions of cells or particles with diameters between 1 and 50 um (microns). Ideally samples should contain 500,000 cells or particles per mL. Sample consumption can be varied between 12 uL/min and 60 uL/min, and so small samples can be analyzed relatively easily. All samples to be run should be non-toxic (given the present instrument siting) and be completely fixed (cells should be dead).
Model No.: Facs-Calibur
Year of manufacture: 2000
Year acquired: 2002
Location: Parsons W118
Specifications: Fluorophores are excited at 488 nm or 635 nm with subsequent emitted fluorescence collected at various wavelengths
Principal scientist: Mark Townley
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