Mol D. Tan

University of Rhode Island

Pharmacy


1993

Mentor: Joanne Curran-Celentano, Associate Professor of Animal & Nutritional Science

Measurement of Serum Fat-Soluble Antioxidant Nutrients as Related to Diet, Body Composition and Hormone Status in Pre-, Peri-, and Post-Menopausal Women

The focus of this study is to measure serum concentration of fat-soluble antioxidant nutrients as related to diet, body composition and hormone status in menopausal women with and without hormone replacement therapy (HRT). Subjects were assigned to one of five groups according to their stage of menopause: (l) Pre-menopausal women; (2) Peri-menopausal and using HRT; (3) Peri-menopausal and not using/never used HRT; (4) Post-menopausal and using HRT; and, (5) Post-menopausal and not using/never used HRT.

One hundred and twenty six women between the ages of 38-55 volunteered as subjects. A fasting blood sample was collected and immediately divided and frozen for analysis. Subjects completed a health history questionnaire and a food frequency questionnaire. Height, weight, and skin fold measurements were recorded.

The serum antioxidants, including Vitamin E, A and beta-carotene have been measured by reverse phase high performance liquid chromatography (HPLC). Data has been collected for: (l) Height, weight, skin fold and body composition; (2) Vitamin E, A and beta-carotene levels (antioxidant nutrients); (3) Cholesterol level of HDL, LDL and TG; and, (4) Estradiol and follicle-stimulating hormone levels are being used to classify the groups. A Food Frequency Questionnaire (FFQ) are being analyzed in Seattle. Once all the analysis is completed, correlations between body composition, dietary intake of antioxidant nutrients, blood values of antioxidant as influenced by hormone status will be made to investigate the relationships that may influence health status of women as they pass through menopause. Data analysis is in progress.

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