University Instrumentation Center

Flow Cytometry at the UIC

What is flow cytometry?

Flow cytometry is a method for the measurement of relative size, granularity, and fluorescence of single cells in a population of cells suspended in a liquid medium. Samples are stained with specific fluorescently-labeled reagents (e.g., antibodies). The labeled cells are illuminated by a 488 or 635 nm laser, and the fluorochrome emission (fluorescent intensity) and light scatter (cell size and complexity) are recorded.

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