The unit has two lasers for exciting fluorochromes: an argon laser, which emits sapphire-colored light at 488 nm, and a red diode laser emitting light at 635 nm. This unit is best suited for the analysis of aqueous suspensions of cells or particles with diameters between 1 and 50 um (microns). Ideally samples should contain 500,000 cells or particles per mL.
When polarized light is passed through a solution containing an optically active compound, the polarized light will rotate clockwise or counter-clockwise. The specific rotation is calculated based on the observed optical rotation by taking into account the cell length and sample concentration.
The University Instrumentation Center (UIC) has developed a series of instrument recommendations for many common instruments based on experience maintaining instruments across many disciplines for many years. The following is a guide and the UIC staff is available for instrument consulting and recommendations for instruments not listed as well as for specialized applications.
The University Instrumentation Center's instruments and services have been utilized by the following Academic courses over the last few years. Partnering with the UIC can add a new dimension to an existing lab section or even present a new approach to teaching familiar concepts. Engaging the students in state of the art complex instrumentation can broaden their knowledge and understanding of the sciences and engineering. Please contact any of the UIC scientists about using our instruments in your lab sections.
We look back on a successful 2015 and we look forward to new opportunities and challenges in 2016.
Throughout 2015, the University Instrumentation Center (UIC) focused on strengthening its value to the university and the local research community. Some highlights of our major accomplishments in 2015 include:
The Zeiss/LEO 922 Omega TEM is a research microscope with accelerating voltages of 120 and 200kV and has magnification from 80X to 1,000,000X with a resolution line of 0.12nm. The in-column energy filter allows researchers to look at unstained or faintly stained materials and tissues. The high resolution objective lens allows the user to tilt a single-grid specimen holder plus or minus 15 degrees.