Zeiss LSM 510 Meta laser scanning confocal microscope
This fluorescence microscope is used primarily for imaging fluorescent probes, conjugates, and proteins in cells and tissues, though it also has non-biological fluorescence applications. It can automatically collect a series of in-focus fluorescence images from thin optical slices at different depths within relatively thick specimens (typically at least 100 um) to create z-stacks. 3-D re-constructions and maximum intensity projections can then be generated from the z-stacks. Multiple fluorophores (2-4 typically, 8 theoretically) excited in the visible wavelength range can be imaged simultaneously. A spectral detector allows fluorophores with overlapping emission spectra to be distinguished from one another.